3.4.1 BugBase Forecast
BugBase analysis is capable of predicting the phenotypes of prokaryotic bacteria in environmental samples. In Figure 6-a, the predominant phenotypes observed in all samples at the end of the experiment were identified as Gram Negative, Stress Tolerant, Oxygen demand (Aerobic, Anaerobic, Facultatively Anaerobic), Gram-Positive, Potentially Pathogenic, Contains Mobile Elements, and Forms Biofilms. The study showed that the abundance of different phenotypes of microorganisms changed at different treatment periods. Following the initial MHWs, an increase in the relative abundance of Potentially Pathogenic and Gram-Positive bacteria was observed in the HA group. After the 5-day recovery, the HB group displayed an increase in the relative abundance of Stress Tolerant and Gram-Positive bacteria. Subsequently, after the second round of MHWs, the HC group showed an increase in the relative abundance of Mobile Elements, Forms Biofilms, Stress Tolerant, and Gram-Positive bacteria. Similar patterns were also observed in the HD group, while there was an elevation in the relative abundance of Anaerobic bacteria at the same time.