Effect of enzyme load on the yield of CG
The influence of the enzyme load on the product yield was investigated
as shown in Fig. 4. The enzyme dosage was varied from 5 % to 30 % of
the total substrate weight. It was found that the yield of CG gradually
increased with the increase of enzyme dosage, likely due to more active
sites, a higher probability of enzyme-substrate collision, and a higher
reaction rate (Yadav & Devendran, 2012). However, when enzyme load was
above 25 %, the yield of CG was almost the same with that of 25 %,
likely due to biocatalyst agglomeration and possible diffusional
problems of substrates at higher enzyme load (Sun & Hu, 2017). So, the
optimal enzyme dosage was selected as 25 %.
Fig. 4. Effect of enzyme load on the yield of CG. Reaction
conditions: the molar ratio of CA to glycerol 1:150, reaction
temperature 80 oC, 86.7 kpa.