Genome annotation
Genome annotation was performed using FGENESH++ v7.2.2 (Softberry; (Solovyev et al., 2006)) on a Pawsey Supercomputing Centre Nimbus cloud machine (256 GB RAM, 64 vCPU, 3 TB storage) using the longest open reading frame predicted from the global transcriptome, non-mammalian settings, and optimised parameters supplied with the Corvus brachyrhynchos (American crow) gene-finding matrix. BUSCO v5.2.2 in ‘protein’ mode was used to assess the completeness of the annotation with the Aves_odb10 lineage on Galaxy Australia.
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