3.1 Genome sequencing and assembly
The genome assembly was 1.48 Gb in length, comprising 1,516 contigs with
a contig N50 of 7.715 Mb (Tables S3-S4). Over 99.99% of raw reads were
retained after quality trimming. The genome had 97.18% complete BUSCOs
[Single copy: 96.4%; Duplicated: 0.7%], 0.5% fragmented BUSCOs
and 2.4% missing BUSCOs. A total of 12,877 predicted genes were used as
evidence for genome annotation from the global transcriptome, which had
94.9% complete BUSCOs [Single copy: 15.0%; Duplicated: 79.9%],
1.3% fragmented BUSCOs and 3.8% missing BUSCOs. After annotation,
30,347 genes were predicted, with an annotation completeness of 82.8%
BUSCOs [Single copy: 82.1%; Duplicated: 0.7%], 7.7% fragmented
BUSCOs and 9.5% missing BUSCOs. The mitochondrial genome was 16,867 bp
in length and was identified on a single contig (Figure S4).