SBI regulated Tfh cell proportion in a dose dependent manner.
In order to validate the in
vivo findings of SBI on lymphocytes, we measured the changes of T and B
cell subsets within R848-induced mice splenocytes cultures subsequent to
SBI treatment. Likewise, SBI treatment successfully rectified the
anomalous proportions of Treg cells, Tfh cells and plasmablast of
R848-induced mice in vitro , yet had no effect on the percentages
of Th1 and Th2 cells (Fig. 3A). These results indicate that SBI
primarily participates in the regulation of Tfh/Treg cell equilibrium.
Moreover, our data showed that SBI inhibited the frequency of Tfh cells
in a dose dependent manner. With the increase of SBI concentration (0-
400μM), the inhibitory ability of SBI on Tfh cells gradually increased
(Fig. 3B).