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\begin{document}

\title{Beyond a single temperature threshold: applying a cumulative thermal
stress framework to plant heat tolerance}



\author[1]{Alicia Cook}%
\author[2]{Enrico Rezende}%
\author[1]{Katherina Petrou}%
\author[1]{Andrea Leigh}%
\affil[1]{University of Technology Sydney}%
\affil[2]{Pontificia Universidad Catolica de Chile}%


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  \date{May 20, 2024}


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\begin{abstract}
Most plant thermal tolerance studies focus on single critical
thresholds, which limit the capacity to generalise across studies and
predict heat stress under natural conditions. In animals and microbes,
thermal tolerance landscapes describe the more realistic, cumulative
effects of temperature. We tested this in plants by measuring the
decline in leaf photosynthetic efficiency
(F\textsubscript{V}/F\textsubscript{M}) following a combination of
temperatures and exposure times, then modelled these physiological
indices alongside recorded environmental temperatures. We demonstrate
that a general relationship between stressful temperatures and exposure
durations can be effectively employed to quantify and compare heat
tolerance within and across plant species and over time. Importantly, we
show how F\textsubscript{V}/F\textsubscript{M} curves translate to
plants under natural conditions, suggesting that environmental
temperatures often impair photosynthetic function. Our findings provide
more robust descriptors of heat tolerance in plants and suggest that
heat tolerance in disparate groups of organisms can be studied with a
single predictive framework.%
\end{abstract}%



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\textbf{Hosted file}

\verb`Cook etal_Plant-cumulative-heat-stress_MainText_v2_20240122.docx`  available at \url{https://authorea.com/users/657177/articles/825673-beyond-a-single-temperature-threshold-applying-a-cumulative-thermal-stress-framework-to-plant-heat-tolerance}

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