4. 4 Mosquito cycles
Anopheles stephensi mosquitoes were maintained as previously
described (Narwal et al. , 2022). To obtain sporozoites,
mosquitoes were allowed to feed on infected Swiss mice, and cages were
kept in an environmental chamber maintained at 190°C with 80% relative
humidity. The development of oocysts was observed on day 14, and
salivary gland sporozoites were dissected between days 18-22
postfeeding.