4. 9 Immunization and challenge experiment
Female C57BL/6 mice (6-8 weeks old) were intravenously injected with three doses of salivary gland sporozoites at an interval of two weeks. Control groups were injected with uninfected mosquito salivary gland debris. Both groups were challenged with 5,000 WT sporozoites 10 post last immunization. The appearance of parasites in blood was monitored by making Giemsa-stained blood smears.