Identification of putative genes that natively inhibit SARS-CoV2
and VSVG entry.
We identified salt inducible kinase 2 and 3 (SIK2 and SIK3) as top
negative regulators of Spike-mNG entry (Figure 3A). These
serine/threonine protein kinases belonging to the AMPK protein family,
and they have been shown to regulate glucose metabolism, mTORC signaling
and hormonal signaling (Bricambert et al., 2010; Csukasi et al., 2018;
Yang et al., 2013; Zhang et al., 2016). However, the mechanism by which
these kinases might inhibit spike-mediated entry is unclear and we have
not validated this finding. Additionally, GALNT11 was identified as a
suppressing VSVG-mNG and VSVG-CRE entry and ALG5 in suppressing VSVG-CRE
(Figure 3B and 3C). GALNT11 is an N-Acetylgalactosaminyltransferase and
is involved in O-linked glycosylation of proteins in the Golgi,
whereases ALG5 is involved in N-linked glycosylation of proteins (Imbach
et al., 1999; Schwientek et al., 2002).