Figure 2: Generation of ACE2 293T and CRE reporter ACE2 293T
cell lines and their characterization. A) 293T cells were transduced
with lentivirus containing ACE2 cDNA for stable ACE2 293T cell line
production. Cells were sorted after 72h of transduction after staining
for ACE2 using AF488 conjugated anti-ACE2 antibody. The histogram shows
ACE2 expression in these cells after sorting, red is the parental 293T
cells and cyan is the ACE2 expressing 293T cells. B) ACE2 293T
cells were transduced with lentivirus containing CRE reporter gene and
cells were selected with blasticidin to get the uniform population of
dsRED expression. C) GFP expression quantified after infection
of ACE2 293T with Spike-mNG and VSVG-mNG or ACE2
293T-GFPfloxed with VSVG-CRE pseudoviruses. Spike-mNG
pseudovirus was concentrated to allow for titers similar to VSVG-mNG.