To compare genes detected by the different screens, diagramed their
subcellular localization and known functions (Figure 5), using
publication and web databases including STRINGDB (string-db.org) and
GeneCards (genecard.org). Broadly, our screens identified genes
affecting viral attachment, the formation of endosomes including Arp2/3,
endosome acidification
ATP6V
genes and expression of downstream reporters.
Figure 5: Top ranked genes (FDR ˂ 0.3) in three different
screens are involved in key elements of the viral life cycle. Schematic
diagram of the viral envelope mediated entry in target cells, endosome
formation, endosome acidification and release of viral genome in the
cytoplasm, reverse transcription and integration and translation of
reporter gene expression. The color of asterisks represents the
corresponding screen condition. Three colors represent that the genes
are present in all three screen conditions. These genes were known to
have an effect on the entry of viral particles and were put in the
diagram based on their function and location in the cell.