To compare genes detected by the different screens, diagramed their subcellular localization and known functions (Figure 5), using publication and web databases including STRINGDB (string-db.org) and GeneCards (genecard.org). Broadly, our screens identified genes affecting viral attachment, the formation of endosomes including Arp2/3, endosome acidification ATP6V genes and expression of downstream reporters.

Figure 5: Top ranked genes (FDR ˂ 0.3) in three different screens are involved in key elements of the viral life cycle. Schematic diagram of the viral envelope mediated entry in target cells, endosome formation, endosome acidification and release of viral genome in the cytoplasm, reverse transcription and integration and translation of reporter gene expression. The color of asterisks represents the corresponding screen condition. Three colors represent that the genes are present in all three screen conditions. These genes were known to have an effect on the entry of viral particles and were put in the diagram based on their function and location in the cell.