Figure 4: Validation of key genes identified in
CRISPR-Cas9 whole genome screening using the one vector system. A) ACE2
staining on the ACE2 KO cells using AF488 conjugated anti-ACE2 antibody
(dark blue) which is similar to unstained (magenta) versus WT ACE2
positive cells (light blue). B) ACE2 staining on the ACE2-293T-CRE
reporter cells following transduction with the ACE2 targeting sgRNA
(dark blue) relative to WT (light blue) and unstained (magenta). C-F)
Effect of mutants on the entry of mNG and Cre reporter viruses. The
error bar represents three biological replicates. One-way ANOVA and
Šídák’s multiple comparisons test were performed to do statistical
analysis between the WT and the mutants. Bar shows mean ± SD (n= 3). *
Denotes p<0.05 for comparison between WT and the mutants.