Conclusion
Overall, this approach provides a new strategy for identifying host
genes influencing viral entry without the confounding complexity of
live-viral screens which produce long gene lists associated with all
aspects of viral pathogenesis and replication. This approach provides a
pathway for increasing the specificity of CRISPR whole genome screens
for identifying host genes contributing to specific steps in viral
infection.
Keywords: Sars-CoV-2, COVID-19, pseudovirus, CRISPR, VSV-G,
screen.