Figure 2: Generation of ACE2 293T and CRE reporter ACE2 293T cell lines and their characterization. A) 293T cells were transduced with lentivirus containing ACE2 cDNA for stable ACE2 293T cell line production. Cells were sorted after 72h of transduction after staining for ACE2 using AF488 conjugated anti-ACE2 antibody. The histogram shows ACE2 expression in these cells after sorting, red is the parental 293T cells and cyan is the ACE2 expressing 293T cells. B) ACE2 293T cells were transduced with lentivirus containing CRE reporter gene and cells were selected with blasticidin to get the uniform population of dsRED expression. C) GFP expression quantified after infection of ACE2 293T with Spike-mNG and VSVG-mNG or ACE2 293T-GFPfloxed with VSVG-CRE pseudoviruses. Spike-mNG pseudovirus was concentrated to allow for titers similar to VSVG-mNG.