2.5 Extraction and evaluation of the solubility of the colorants
After 14 days of fermentation, each culture broth was separated from the mycelial mass by filtering through ordinary filter paper. The mycelial mass was washed with hexane (20 mL), ethyl acetate (20 mL) and methanol (20 mL). Solvents were added sequentially to the cultures and vigorously shaken in an orbital shaker (100 rpm/60 min). Extracts were filtered through filter paper for the removal of cell debris. The culture filtrates were subjected to successive extractions. The culture filtrate and solvent (hexane and ethyl acetate) were placed in a separating funnel (1:1, v/v) and mixed well. After separation, the solvent fraction was collected.