Overall, research on the seminal plasma proteome has the potential to yield important insights into male reproductive biology and the mechanisms underlying male infertility and other reproductive disorders. As a vulnerable species, the reproduction of giant pandas has always been a matter of great concern. Scientists have been studying ways to increase the breeding rate of captive giant pandas to ensure their population’s health and sustainability. By establishing a dataset of the giant panda seminal plasma proteome, and identifying specific proteins and biomarkers associated with reproduction, researchers may be able to develop new diagnostic and therapeutic approaches to improve male panda reproductive health.