Animals

All animal experiments were carried out on adult male C57BL/6J mice or male and female CXCR1EGFP mice aged 8-12 weeks old with an average body weight of 23 ± 3g. C57BL/6J Mice were sourced from the Animal Resources Centre (Western Australia) and CXCR1EGFP were a gift from Dr Andrew Jobling (University of Melbourne) Mice were housed in groups of up to five per cage on a 12:12h light/dark cycle, with food and water provided ad libitum . All procedures, monitoring and reporting was performed in accordance with standard protocols approved by the University of Melbourne Animal Ethics Committee (Ethics ID # 1814589.4). All experimental work involving animals followed the ARRIVE guidelines for animal research .