3.5 Functional enrichment of PSGs
GO enrichment analyses showed that the candidate PSGs were significantly overrepresented and enriched into GO categories such as: metabolic process (GO: 0008152), protein metabolic process (GO: 0019538), single-organism process (GO: 0044699) (Table 5). These categories appeared to be biologically relevant to energy metabolism.
By comparing the GO annotations enriched in different specieslow, we found some GO terms enriched more PSGs in each species. For the PSGs in R. siamensis , we listed six over-represented GO categories, and some of them related to catalytic activity and metabolic processes. For R. episcopus , three significantly enriched GO categories in terms of single-organism process, catalytic activity, and single-organism process enriched more PSGs. We found three GO categories enriched more PSGs compared with other categories in R. marshalli . The PSGs identified in R. rex were mainly enriched in GO terms of molecular function, single-organism process, catalytic activity, and protein metabolic process.