2.5 | Data analysis
At the end of the experiment, the measured data were entered and organised in Microsoft Excel, and the organised data were imported into R and Graphpad Prism 8.4.3 for graphing. First, the data were imported into R language with ”agricolae” package for multiple comparisons, p<0.05 for significant differences, and then Graphpad Prism 8.4.3 comes with the data analysis function to check the significance of the results and make graphs, the significance of the graphs with ”*”, ”*” for significant differences, ”*” for significant differences and ”*” for significant differences. ”*” is p≤0.05, ”**” is p≤0.01, ”***” is p≤0.001, ”****” is p≤0.0001. Soil nutrient content and pH data were plotted as box-and-line plots using Graphpad Prism 8.4.3, and images were spliced and labelled with axis information and significance of differences using Adobe Illustrator software. The data of soil microorganisms MBC and MBN were plotted as scatterplot and trend line using Graphpad Prism 8.4.3 software, and the images were spliced and labelled with axis information and significance of difference using Adobe Illustrator software, and the significance was expressed in the same way as above. Finally, the data were imported into R to analyse the correlation between soil factors and soil microbial MBC and MBN contents. The R package piecewiseSEM was used to analyse the data and plot the segmented structural equation model, and Graphpad Prism 8.4.3 was used to calculate the coefficients. Graphpad Prism 8.4.3 was used to calculate the effect coefficients and plot the effect-effect bar charts, and Microsoft Office PowerPoint was used to draw and beautify the structural equation model graphs.