Figure 5. Cryo-EM structure of two mvACE2 bound to upRBD in SARS-CoV-2 mink S glycoprotein. (A) Cryo-EM map of upRBD mink S glycoprotein-mvACE2 complex at 3.36 Å. (B) Cryo-EM map of the Mink S glycoprotein RBD with mvACE2 after local refinement at 3.82 Å [PDB: 8T23]. (C) Contacting residues located less than 4 Å are labeled, and lines indicate the interactions. Hydrogen bonds, ionic, contact, pi-cation, and pi-pi stacking interactions are indicated by green, cyan, magenta, red, and blue, respectively. (D-F) Comparison of the close-up view of the RBD-ACE2 interface between the interface of the trimeric spike cryo-EM structure (top panel) and of the Y453F RBD-ACE in the x-ray crystal structure [PDB: 7F5R] (bottom panel). The key interacting residues are labeled.