Figure 4. Cryo-EM structure of mvACE2 bound downRBD in SARS-CoV-2 mink S glycoprotein. (A) Cryo-EM map of downRBD spike glycoprotein-mvACE2 complex at 3.75 Å resolution. (B) Cryo-EM map of the Mink S glycoprotein RBD with mvACE2 after local refinement at 3.62 Å [PDB: 8T25] (C) Contacting residues less than 4 Å are labeled, and lines indicate the interactions. Hydrogen bonds, contact, pi-cation, and pi-pi stacking interactions are indicated by green, cyan, magenta, red, and blue, respectively. (D-F) Close-up view of the RBD-ACE2 interface, with key interacting residues labeled.