3 | RESULTS
3.1 | RAD-seq dataset
The RAD-seq genome library of 108 individuals of Sinocyclocheilusindividuals yielded approximately 4.1 million reads per individual on average after splitting and filtering the original data. The proportion of bases with a mass value greater than 20 in all reads to the total reads length was more than 96.15%. Among them, 92% of reads were on average mapped to the reference genome of Sinocyclocheilus graham(Yang et al., 2016). After variant calling and filtering, a total of 26,318,419 SNPs were identified from 108 individuals ofSinocyclocheilus .