Fig.12(A)Representative images of wound tissue sections stained by
Masson in different treatment groups on day 3 (Scale bar ¼ 1 mm and 50
μm). (B) Quantification of the collagen content around laser effect zone
in different treatment groups on day 3 (n ¼ 3). (C) Quantification of
the collagen fractional area around laser effect zone in different
treatment groups on day 3 (n ¼ 3).
As shown in Fig.13(A), the wound direction of the four groups of samples
on the 3rd day of healing and the distribution of
different types of collagens around the wound were separately labeled.
It can be observed that the proportion of two types of collagens in the
90° laser group was the most prominent, up to 2.68, and the COL-1 fiber
content was significantly higher than that in the other three groups (as
shown in Fig.13(C)). This indicated that the tissue of the samples in
this group was relatively stiff after the first laser welding, but the
strength was higher, which met the standard of good healing. According
to Fig.13(B), the ratio of COL-1 fibers and COL-3 fibers of samples
under 90turned was also the highest. However, the ratio of COL-1 and
COL-3 fibers in the samples in two laser groups with incident angles
were significantly lower than that in the control group, indicating that
although the collagen proliferation of the samples in these two groups
was higher than that in the control group without any operation, while
COL-3 fiber was the collagen that
prolifically proliferated in 60 laser and 30 laser groups, the
tissue was softer, and the elasticity was significantly higher than that
of the 90° laser group at the 3rd day. After the first
laser welding, the higher elasticity of wound tissue indicated that the
samples were not prone to scar, and the thermal damage caused by laser
was also relatively low under 60 laser and 30 laser.