3.3 Protein-protein interaction analysis highlights glycoprotein
mediated biological pathways in HGGs
A list of significantly upregulated proteins from HGG was used to
perform protein-protein interaction analysis. PPI analysis showed the
significant upregulated proteins to be mapped to four different
biological pathways in HGG. These pathways include; focal adhesion (n =
12), N-glycan biosynthesis (n = 3), ECM receptor interaction (n = 10)
and complement and coagulation cascades (n = 9) (Figure 3) .
Proteins RPN1, RPN2 and DAD1 are the subunits of OST complex which is
responsible for glycosylation. Further, FN1, FGA, FGB, FGG, VTN and
collagens are the crucial molecules related to ECM and have shown high
interaction with each other. The trend of few significant proteins was
shown in the form of violin plots (Figure 3).