3.2 Pathway enrichment analysis showed epithelial mesenchymal
transition pathway to be highly enriched in high grade gliomas
Gene set enrichment analysis of identified proteins resulted in enriched
pathways associated with glioma pathobiology ranked according to their
normalized enrichment score (NES) along with the enriched proteins in
each pathway (Figure 2D, Figure S1) . The top 5 enriched
pathways in HGG include, epithelial mesenchymal transition (NES = 1.77),
focal adhesion (NES = 1.48), regulation of actin cytoskeleton (NES =
1.41), MTORC 1 signaling (NES = 1.28) and hypoxia (NES = 1.16)
(Figure S2 ). Further GSEA with gene ontology functional
database for cellular component using WebGestalt showed maximum number
of proteins in HGG were mapped to extracellular matrix, blood
microparticle and ER lumen respectively with FDR less than 0.05
(Figure S3 ).