3.3 Protein-protein interaction analysis highlights glycoprotein mediated biological pathways in HGGs
A list of significantly upregulated proteins from HGG was used to perform protein-protein interaction analysis. PPI analysis showed the significant upregulated proteins to be mapped to four different biological pathways in HGG. These pathways include; focal adhesion (n = 12), N-glycan biosynthesis (n = 3), ECM receptor interaction (n = 10) and complement and coagulation cascades (n = 9) (Figure 3) . Proteins RPN1, RPN2 and DAD1 are the subunits of OST complex which is responsible for glycosylation. Further, FN1, FGA, FGB, FGG, VTN and collagens are the crucial molecules related to ECM and have shown high interaction with each other. The trend of few significant proteins was shown in the form of violin plots (Figure 3).