3.2 Pathway enrichment analysis showed epithelial mesenchymal transition pathway to be highly enriched in high grade gliomas
Gene set enrichment analysis of identified proteins resulted in enriched pathways associated with glioma pathobiology ranked according to their normalized enrichment score (NES) along with the enriched proteins in each pathway (Figure 2D, Figure S1) . The top 5 enriched pathways in HGG include, epithelial mesenchymal transition (NES = 1.77), focal adhesion (NES = 1.48), regulation of actin cytoskeleton (NES = 1.41), MTORC 1 signaling (NES = 1.28) and hypoxia (NES = 1.16) (Figure S2 ). Further GSEA with gene ontology functional database for cellular component using WebGestalt showed maximum number of proteins in HGG were mapped to extracellular matrix, blood microparticle and ER lumen respectively with FDR less than 0.05 (Figure S3 ).