2.5 Inactivation kinetic analyses
The inactivation kinetic analysis of gallic acid and quercetin towards
SARS-CoV-2 Mpro was
performed in black 96-well plates. The enzyme was preincubated with
inhibitors at varying concentrations and durations, and then the
reaction was started with a constant substrate concentration. The
natural logarithm of the residual activity of SARS-CoV-2
Mpro was plotted against the preincubation time using
Graphpad Prism 7.0. Detailed information was as described in the
reported literature24,27.