2.5 Inactivation kinetic analyses
The inactivation kinetic analysis of gallic acid and quercetin towards SARS-CoV-2 Mpro was performed in black 96-well plates. The enzyme was preincubated with inhibitors at varying concentrations and durations, and then the reaction was started with a constant substrate concentration. The natural logarithm of the residual activity of SARS-CoV-2 Mpro was plotted against the preincubation time using Graphpad Prism 7.0. Detailed information was as described in the reported literature24,27.