Fig. 2. Amplification curves of each target gene by serial dilutions between synthetic DNA (column A) and plasmid DNA (column B) standards.Standards were diluted by 10 times for each step from 108 to 101 copies per μl. NC: negative control. Rn is the fluorescence of the reporter dye divided by the fluorescence of a passive reference dye; i.e., Rn is the reporter signal normalized to the fluorescence signal of Applied Biosystems™ ROX™ Dye.