16S rRNA gene ITS mcrA pmoA nifH nosZ archaeal amoA bacterial amoA nirS nirK nxrB norB phoD
Stage: denaturation
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
2 min at 95 ºC
Stage 2: annealing temperature 53 ºC 58 ºC 50 ºC 56 ºC 55 ºC 55 ºC 53 ºC 55 ºC 58 ºC 57 ºC 57 ºC 57 ºC 58 ºC
Stage 3: melting temperature 60 ºC 60 ºC 50 ºC 60 ºC 50 ºC 50 ºC 60 ºC 60 ºC 60 ºC 60 ºC 60 ºC 55 ºC 60 ºC
Stage 4: cooling 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC 4 ºC