Morphology of parasporal inclusions
Cultures sporulated from 5 to 6 days were observed using a phase-contrast microscope to morphologically identify paraspore inclusions (Chai et al., 2014). Biochemical and phenotypic characterization and identification of 190 B. thuringiensisisolates were performed based on esculin hydrolysis, lecithinase, hemolytic activity, and motility activity (El-Kersh et al., 2016).