Sequence analysis and phylogeny
SeqMan software (DNASTAR Inc., Madison, WI, USA) was used to assemble
the obtained sequences, which were uploaded to the NCBI database for
blast. The ClustalW method (MEGA X, www.megasoftware.net) was used for
the sequence comparison using the neighbor-joining method to construct
phylogenetic trees after estimation of genetic distance (Kimura
two-parameter method). A bootstrapping test was performed 1,000 times.