2.2 Comparison of metabolic activity and cell structure of different types of chlamydospores
FUN-1 staining showed that the dormant spores could only see green fluorescence after the simultaneous excitation of 488 nm and 633 nm laser (Figure 2A), while non-dormant spores showed orange fluorescence. Non-dormant spores can be superimposed under both fluorescence conditions, while dormant spores cannot be superimposed, suggesting that non-dormant spores have stronger metabolic activity than dormant spores (Figure 2B). DAPI staining results showed that the nuclei of resting spores were regular, round and dense, showing bright blue fluorescence (Figure 2C & 2D). Non-dormant spore nucleus materials emit weak fluorescence, showing blue circles. CFW staining showed that non-dormant spores could produce strong yellow-green fluorescence, while dormant showed weak yellow-green fluorescence (Figure 2E & 2F), indicating that there was a significant difference between the binding strength of cell walls and CFW dye.