2.2 Comparison of metabolic activity and cell structure of
different types of chlamydospores
FUN-1 staining showed that the dormant spores could only see green
fluorescence after the simultaneous excitation of 488 nm and 633 nm
laser (Figure 2A), while non-dormant spores showed orange fluorescence.
Non-dormant spores can be superimposed under both fluorescence
conditions, while dormant spores cannot be superimposed, suggesting that
non-dormant spores have stronger metabolic activity than dormant spores
(Figure 2B). DAPI staining results showed that the nuclei of resting
spores were regular, round and dense, showing bright blue fluorescence
(Figure 2C & 2D). Non-dormant spore nucleus materials emit weak
fluorescence, showing blue circles. CFW staining showed that non-dormant
spores could produce strong yellow-green fluorescence, while dormant
showed weak yellow-green fluorescence (Figure 2E & 2F), indicating that
there was a significant difference between the binding strength of cell
walls and CFW dye.