DNA extraction, library construction, and sequencing
Genomic DNA was extracted from silica-dried leaves using a modified CTAB
protocol (Porebski, Bailey, & Baum, 1997). The 52 LCN genes were
amplified by PCR using the protocol of Jiang et al. (2022), and the DNA
library was constructed following de Sousa et al. (2014). After
obtaining fragments of 300–400 bp, PCR was performed on the indexed
samples, and the concentrations of the amplified library were measured
using a NanoDrop 2000c (Thermo Fisher Science, USA). Gene enrichment and
hybridization reactions were performed following the protocol of de
Sousa et al. (2014), and the pooled samples were high-throughput
sequenced using the Illumina MiSeq platform to generate 150-bp
paired-end reads.