2.5 Antioxidant enzyme activities and histochemical staining
SOD, POD, and CAT activities, and ROS
(H2O2 and
O2−) levels were estimated using
colorimetric assay kits (Comin, Suzhou, China). After 8h of heat
exposure, the presence of H2O2 and
O2− were stained with DAB and NBT,
respectively.