Histoarchitechture studies of cancer tissues
Breast tumor tissue and its corresponding surrounding tissues were embedded in paraffin, serially sectioned at 5 μM by an automated cryostat slicing machine (Leica Biosystems), stained with eosin and hematoxylin (Harris), and observed under a microscope (Nikon, Eclipse LV100, magnification 20X) to study the tissue histoarchitecture.