Estimation of Malondialdehyde (MDA) Levels
The MDA was estimated both from liver tissue and serum samples. Tissue was homogenized (10 % w/v) in the ice-cold phosphate buffer (0.1 mol /L, pH 7.4) and the homogenate was centrifuged at 10,000 rpm at 4ºC for 10 min. The MDA assay was conducted using the supernatant following the protocol (25). To chelate iron and reduce its interference in peroxidation reaction of unsaturated fatty acid, 1 mM EDTA was used in the reaction mixture. To reduce the interference caused by a yellow-orange colour produced by some carbohydrates, the reaction mixture was heated at 80ºC instead of 100ºC. Finally, the MDA was measured and calculated utilizing the molar extinction coefficient of MDA (1.56 x 105 cm2/ mmol).