Evaluation of general toxicity
Serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), NPSH and MDA were measured from the rat serum treated with DAS, Chal, and DAS + Chalcone for 24, 48 and 72 hours. SGPT and SGOT were measured by standard protocol with the assay kits (Ranbaxy, India or other reputed company). Total protein from serum was measured following the standard protocol (27).