Statistical analysis of relation between Cancer manifestation
and this disease causing factors
The protein density and mRNA density (in surrounding and tumor) from
western blot and RT-PCR data suggests that both PD and MD of 1E1 and
HIFα are significantly increased in the tumor groups (Table 1 and Fig
1). Present correlation study suggests that tissue MDA level is
positively associated with SULT1E1 tissue expression. The 1E1 is an
adaptive enzyme that catalyze the inactivation of E2 by forming E2S.
More oxidative stress as evident in high MDA level has some initial role
in active E2 nullification. MDA is negatively associated with HIFα
expression. At the initial phase of MDA related stress response has more
adaptive responses of lower HIFα. But in breast cancer tissue it is
noticed that, at higher disease state with prolonged oxidative stress
exposure and high MDA level HIFα is increased significantly and Nrf2 is
shown to positively associated with HIFα induction. NPSH and uric acids
are regarded as the endogenous soluble antioxidants those may have some
protective role against the oxidant-stress-induced 1E1 regulations and
Nrf2, HIFα induction. Present results and correlation data suggest that
NPSH and uric acids are negatively correlated with HIFα expression.
Lower antioxidant level augments the HIFα level (Table-2). The data from
this table are represented from DAS and chalcone related studies shown
in the figure from in fig 4, 5 and 6. Table 3 suggests that in control,
DAS and Chalcone groups, NPSH and 1E1 are found to be associated. When
in control and in DAS these are positively associated in Chal- group
NPSH and 1E1 are shown to be negatively associated. No significant
association was noticed in DAS and Chalcone groups.
Table 4 suggests that, in case of time-based exposure NPSH and 1E1 and
1E1 are found to be positively associated and 3 and 24 hrs of exposure.
No significant relation was noticed in 5 hrs exposure. New sample size
and internal variability may be the cause for this.