In –vivo (24, 48, 72 hours treatment)
Animals were divided in 3 groups, Group 1-control, Group 2-Diallysulfide
(DAS), 80 mg DAS/100gm b.w., Group 3- Chalcone, 4 mg/100gm b.w., Group
4- Combination of DAS (80 mg) and Chalcone (4mg). The animals were
treated with the drug or drug combination by gavages. DAS and chalcone
were solubilized in 0.1% ethanol (Ethanol+water) to get the required
concentration. The current dose of DAS and chalcone was screened after
consultation with some previously published reports (17-19). In
addition, some dose response studies were performed in our lab to
validate the selected dose. The present dose demonstrated significant
responses on some genes expression like SULT1E1 and this dose did not
initiate any toxicity which was evaluated from animal body weight, organ
weight/body weight ratios, liver and kidney function test and
haematology or other toxicity studies. Control group was given same
amount of 0.1 % ethanol solution as. Different groups had 3-5 animals
per group and animals were serially sacrificed at 24, 48, 72 hours after
drug administration, a second dose was given to a single animal at 72
hour and was sacrificed on the 5th day of the
experiment i.e. at 120 hours or after 48 hours of the second dose.