In vitro experiment
The DAS group expressed SULT1E1 more than the control group during the in-vitro therapy, and this expression gradually increased over time. The chalcone group had less SUT1E1 expression than the control and gradually decreased with increasing time. Whereas the combination group had highest expression at the first 3 hours of incubation, decreased at 5 hours and remain undetected at 12 hours of incubation (Figure-4b). However, 3hr DAS+Chal treated tissue showed the best result with highest level of SULT1E1 and then gradually decreased in 5 hr and least is found in 12 hr. Thus, DAS has direct effect on the expression of SULT1E1 and Chalcone has some moderate effect.