2.4 Plasma Cytokine Detection Assay
Neat plasma from naïve vaccinees and convalescent vaccinees were run in duplicates to measure 20 T cell cytokines using human Milliplex map cytokine assay kit (Millipore, Billerica, MA). The samples were acquired in a Bio-Plex 200 system (Bio-Rad, Hercules, CA) and cytokine concentrations were calculated using Bio-Plex manager software with a five-parameter (5PL) curve-fitting algorithm applied for standard curve calculation.