2.1 Study Design and Sample Collection
Peripheral venous blood was collected from 40 donors, all of them were vaccinated with double dose of inactivated SARS-CoV2 vaccine and out of 40 donors, 20 donors experienced breakthrough infection during delta wave (convalescent vaccines), the rest 20 donors did not have any prior history of SARS-CoV2 infection or did not have any breakthrough infection (naïve vaccinees). The blood collection was performed after 4-6 months of the completion of the second dose. To understand the immune response after SARS-CoV2 infection blood was collected from 20 convalescent patients at least after 1 month of their testing negative of SARS-CoV2. The details of the donors are given in Table 1. These convalescent patients were also vaccinated and tested positive during the delta wave of the SARS-CoV2 virus. The details of the sequence deposited at the time of infection is available at the following link https://inda.rcb.ac.in/insacog/statreportzonelineagegraph. PBMC was isolated from blood, resuspended in CS10 media (Stem cell Technologies), and stored in liquid nitrogen. Plasma was stored at -80℃ till further use.
2.2 Institutional Review Board Statement: The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Institutional Human Ethics Committee, Institute of Life Sciences. The Institutional Ethics Committee (IEC)/Institutional Review Board (IRB) reference number is 106/HEC/2021.
Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.