Hydrophobic Interaction Chromatography
The overall hydrophobicity of the BsAbs was assessed by hydrophobic
interaction chromatography (HIC-HPLC). Samples were diluted to 1mg/ml in
PBS and injected onto a TSKgel Butyl-NPR HIC column (Tosoh Cat.#
0014947) preequilibrated in 25mM potassium phosphate, 1.5M ammonium
sulfate, pH 6.8. Samples were eluted with a 20-minute linear gradient
into 25mM potassium phosphate, pH 6.8, 20% isopropanol.