Differential Scanning Calorimetry Analysis
Thermal stability of the BsAbs was assessed by DSC. Samples were prepared at 1 mg/mL in PBS. Thermal transition of each antibody was measured using an automated MicroCal PEAQ-DSC (Malvern Panalytical, UK) in a scan rate of 1°C/min for the temperature range of 20-110°C, after equilibration at 20°C for 3-min. Thermograms for each sample were buffer referenced and baseline subtracted.