T-Cell Bio-energetic Profile
Surrogate markers of oxidative phosphorylation and glycolysis were
measured by flow cytometry using the following reagents according to the
manufacturer’s instructions. MitoTracker Green FM and MitoTracker Red FM
were from Invitrogen-Molecular Probes.
2-(N-(7-nitrobenz-2-oxa-,1,3-diazol-4-yl)amino)-2-deoxyglucose (2NBDG)
was from Life Technologies. L(+)lactate concentration was measured in
the supernatants with a Lactate Assay Kit (Sigma-Aldrich).