Figure 1. Identification and responsiveness to IL-7 of circulating Treg subsets. (A ) Representative FACS plots showing the gating strategy to identify Treg (CD4+CD25highFOXP3+CD127low) and naïve and memory subsets based on the expression of CD62L, CD45RA and CD95. (B ) Graph shows percentage of each of Treg subset from 16 subjects (C ) Representative FACS plot (left) showing the expression of CD127 in Treg subsets compared to conventional CD4+ T cells and B cells, and graph representing data from 6 subjects (right). (D ). Representative FACS plot (left) showing STAT-5 phosphorylation in Treg subsets compared to conventional CD4+ T cells and B cells after 10 minutes incubation with 10ng/ml of rh-IL-7 (left) measurements from 6 subjects (right). (E ) Expansion curve of bulk Treg cultured with medium (Ctr), IL-2 (100 UI/ml), IL-7 (10ng/ml), and combination of IL-2 and IL-7. (n=3).