2.4 | Twitching motility rate calculation and preparation of time-lapse video
After 10 h of incubation of R. solanacearum strains, the Petri plates containing R. solanacearum F1C1 cells were observed for 12 h 20 mins under Life technologies EVOS FL inverted microscope with 40X magnification using the 4/10 PH condenser annulus. During observation, photomicrographs were taken at an interval of every 5 min in JPEG format which was later compiled to create a time-lapse video.
Three random points at the margin of the spotted area were marked and the displacement from these points at each hour was taken and averaged out. The data obtained were plotted on a scatter plot against time to determine the line of best fit that best explains the rate of twitching motility.