S5 methylation and EPB41L3 were related to the severity of SIL
Median S5 methylation (Figure 2 ) increased proportionally (Cuzick test for trend was significant, P < 0.001) with histological diagnosis: 1.31 in histology Negative (n=172), 2.34 in LSIL (n=297), 12.7 in HSIL (n=399), 18.8 in cancer (n=70). Figure 3shows similar pattern of methylation levels increasing by lesion severity for EPB41L3 (Cuzick test for trend was significant, P< 0.001).
Furthermore, to assess if there was a significant difference between HPV16/18-negative and HPV16/18-positive women stratified by SIL status, we consider the methylation of human gene EPB41L3 in all samples.Figure 4 shows that there was very no significant difference between the < HSIL+ HPV16/18-positive and the < HSIL+ HPV16/18-negative samples (P = 0.14).