Fig.
6. Ribosomal fractions of ΔybeX contain distinct 16S rRNA and 17S
pre-rRNA fragments. (A ) Sucrose gradient profiles of WT andΔybeX . The strains were grown in LB medium at 37°C for 2 hours
after the OD600 reached 0.3. 10-30% sucrose gradients
were used for sedimentation. The gradient profiles are representative of
four independent experiments. (B -F ) Northern blot
hybridization of the same membrane using different Cy5-labelled
oligonucleotides (see Table S3 and Fig. 5A). Truncated ribosomal RNA
species are annotated as “trunc.”. The Cy5-labelled fluorescence
oligonucleotides are 16S 3‘-end, 16S (16S EUB), 17S 3‘-end, 17S
5‘-end, 23S (23S h25) specific, and each probe indicated under panels
B-F.