Total RNA Purification using hot phenol extraction
The strains were grown in LB at 37°C. 10-12 mL of cell culture were transferred to a 15 mL Falcon tube, pelleted via centrifugation at 8000xg for 3-4 minutes, snap-frozen in liquid nitrogen, and stored at -80°C until RNA purification. Total RNA was purified with hot phenol-chloroform extraction, as described previously (Kasari et al. , 2013).