Total RNA Purification using hot phenol extraction
The strains were grown in LB at 37°C. 10-12 mL of cell culture were
transferred to a 15 mL Falcon tube, pelleted via centrifugation at
8000xg for 3-4 minutes, snap-frozen in liquid nitrogen, and stored at
-80°C until RNA purification. Total RNA was purified with hot
phenol-chloroform extraction, as described previously (Kasari et
al. , 2013).