2.5.1 Biocompatibility
To support the growth and proliferation of cells in wound tissue,
biocompatibility of hydrogel should also be tested. The biocompatibility
of hydrogel was evaluated by direct live/dead staining assay and MTT
assay on HaCaT cells. As shown in figure 4A and 4B, different
concentrations of hydrogel had no negative effect on cellular viability
and proliferation. Most cells are alive (green fluorescence) in both the
control and hydrogel group, with only a few dead cells present (red
fluorescence), indicating that the hydrogel have satisfactory
cytocompatibility. As wound dressings inevitably come in contact with
blood, hemolysis assay also was conducted to further evaluate the
hemocompatibility of the hydrogels. As shown in Figure 4C, incubation of
hydrogel or THB@ANVs with the red blood cells did not result in any
apparent hemolysis. The results above suggest that the THB@ANVs
delivered hydrogel showed good biocompatible, non-hemolytic, and
suitable for wound cell growth and proliferation.