4.6 Histopathological analyses
Skin tissues were fixed with 10% formaldehyde for 48 h and then embedded in paraffin and sectioned at a thickness of 4 μm. The samples were then stained with Hematoxylin & Eosin (H&E), Masson’s trichrome or immunofluorescence. They were observed by a pathology slide scanner for the general assessment of wound regeneration.