4.1 Chemical reagents and materials
Dulbecco’s Modifed Essential Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Life Technologies). Phosphate buffered saline (PBS), penicillin, streptomycin, IL-10, IL-6, TNF-α ELISA kits, and DiO were purchased from Thermo Fisher Scientifc. BCA protein assay kit, calcein/PI cell viability assay kit, SDS-PAGE gel quick preparation kit, QuickBlock™ western, EdU cell proliferation kit (488 nm), MTT cell proliferation and cytotoxicity assay kit, crystal violet, phenylmethanesulfonyl fluoride (PMSF), ethylene diamine tetraacetic acid (EDTA), and RIPA lysis buffer were purchased from Beyotime Biotechnology. Carboxymethyl chitosan (molecular weight 240 KDa, degree of deacetylation greater than 90%, degree of amino substitution 90%) were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. 3M Transparent film Dress (1624W) were purchased from 3M™ Tegaderm™.
All the chemicals were used as received without further purification. 5-(4-(diphenylamino)phenyl)thiophene-2-carbaldehyde and 1-(2-hydroxyethyl)-4-methylpyridin-1-ium bromide were synthesized according to a literature method.