Figure 2. In vitro wound repair promoting experiment of
THB NPs, ANVs and THB@ANVs. (A)In vitro wound scratch
assay on HaCaT cells at 0 h, 12 h, and 24 h. Scale bar: 500 μm. (B)
Effect of THB NPs, ANVs or
THB@ANVs on chemotaxis of HaCaT cells by Transwell. Scale bar: 100 μm.
(C) EdU incorporation assay of HaCaT cells incubated with THB NPs, ANVs
or THB@ANVs. (D) Cell proliferation of THB NPs, ANVs or THB@ANVs on
HaCaT cells using MTT (n = 3). Macrophages were treated with LPS
(1μg/ml) and levels of (E)TNF-α, (F) IL-6, and (G)IL-10 were assessed by
ELISA after incubation with THB NPs, ANVs or THB@ANVs (****, p
< 0.0001, n = 3).
EdU incorporation and MTT staining assay were used to analyze their cell
proliferation promoting effects. The number of EdU-positive cells
increased after treatment with ANVs and THB@ANVs, indicating an increase
in DNA replication levels (Figure 2C). And the number of cells in MTT
also significantly increased in a dose-dependent manner (Figure 2D).
Both of them prove that the ANVs and THB@ANVs still retain the function
of ADSCs in promoting cell proliferation. The effect of ANVs and
THB@ANVs on other types of wound repair related cells is similar, which
were detailed in Figure S9 and S10. No statistical difference was
observed between ANVs group and THB@ANVs group, indicating that the
addition of AIE molecules did not adversely affect the proliferation and
migration of cells.