2.6.2 Histological analysis
During the remodeling phase, collagen deposition can indicate the integrity of skin repair, with its ability to improve the tensile strength and epidermal integrity of the tissue. To further find the healing effect of the hydrogels on the wound tissues, the representative H&E and Masson’s trichrome staining on days 7 and 14 were carried out on the dissected wound tissues (Figure 6A). ANVs can enhanced collagen deposition on the regenerated tissue sites. The quantified wound macroscopic observations were in accordance with the closure rates. This also confirmed the in vitro cell experiment results. Higher expression of collagen in scald wound area was observed in ANV hydrogel and THB@ANVs hydrogel group at day 7 and 14. Both data showed that the wounds heal of THB@ANVs hydrogel group were significantly enhanced.