2.3.1 Cell proliferation and migration promoting effects
ADSCs and its natural extracellular vesicles (EVs) have been reported to show positive impact in promoting wound healing.[35,36] The ANVs prepared in this work have similar components to ADSCs, so it is also possible to exhibit similar functions. Using HaCaT cells as a model cell type, cell scratch test and Transwell assay were employed to investigate the migration promoting effects of ANVs and THB@ANVs. The in vitro scratch wound model is a straightforward and reproducible approach for studying cells migration and wound closure. According to the results (Figure 2A), ANVs and THB@ANVs can significantly enhance migration of HaCaT cells from the edge of the scratch compared to the control group after 24 h. More HaCaT cells in the upper Transwell chamber were observed to pass through the membrane into the lower chamber when ANVs or THB@ANVs were added (Figure 2B), which is consistent with the results of cell scratch test.