4.2.3 Preparation of THB@ANVs
After incubated 100 μg/mL 400 nm ANVs with THB at 4 ℃ for 30 min, the
THB@ANVs was then reconstructed by repeatedly extruding through a 200 nm
PCTE membrane of liposome extruder. The THB@ANVs can be purified by
centrifugation at 10000 xg for 30 min and then dialysis with 100 kDa
ultrafiltration tube.