4.4.7 Antibacterial experiment of hydrogel
The 2 cm2 sterile hydrogels were soaked in 180 μL
sterilized PBS for 3d, then 20 μL bacterial suspension
(1×107) was added to incubation with each hydrogel for
30 min. The mixtures were irradiated with white light at a power density
of 100 mW/cm2 for 10 min. They were then diluted 10
times with saline and vigorously mixed in a centrifuge tube. Next,
further diluted the mixture by a 1:100 ratio, and take 100 ul of the
resulting solution to spread on Luria-Bertani agar plates. The plates
were kept at 37 °C for 14~18 h in an incubator, followed
by photography and cell counting. The tests were repeated three times
for each group, and the results were expressed as killing rate (%):
Killing rate (%) = (Cell count of control-Cell count with
hydrogel)/Cell count of control×100%