2.3.1 Cell proliferation and migration promoting effects
ADSCs and its natural extracellular vesicles (EVs) have been reported to
show positive impact in promoting wound
healing.[35,36] The ANVs prepared in this work
have similar components to ADSCs, so it is also possible to exhibit
similar functions. Using HaCaT cells as a model cell type, cell scratch
test and Transwell assay were employed to investigate the migration
promoting effects of ANVs and THB@ANVs. The in vitro scratch wound model
is a straightforward and reproducible approach for studying cells
migration and wound closure. According to the results (Figure 2A), ANVs
and THB@ANVs can significantly enhance migration of HaCaT cells from the
edge of the scratch compared to the control group after 24 h. More HaCaT
cells in the upper Transwell chamber were observed to pass through the
membrane into the lower chamber when ANVs or THB@ANVs were added (Figure
2B), which is consistent with the results of cell scratch test.