Figure 2. In vitro wound repair promoting experiment of THB NPs, ANVs and THB@ANVs. (A)In vitro wound scratch assay on HaCaT cells at 0 h, 12 h, and 24 h. Scale bar: 500 μm. (B) Effect of THB NPs, ANVs or THB@ANVs on chemotaxis of HaCaT cells by Transwell. Scale bar: 100 μm. (C) EdU incorporation assay of HaCaT cells incubated with THB NPs, ANVs or THB@ANVs. (D) Cell proliferation of THB NPs, ANVs or THB@ANVs on HaCaT cells using MTT (n = 3). Macrophages were treated with LPS (1μg/ml) and levels of (E)TNF-α, (F) IL-6, and (G)IL-10 were assessed by ELISA after incubation with THB NPs, ANVs or THB@ANVs (****, p < 0.0001, n = 3).
EdU incorporation and MTT staining assay were used to analyze their cell proliferation promoting effects. The number of EdU-positive cells increased after treatment with ANVs and THB@ANVs, indicating an increase in DNA replication levels (Figure 2C). And the number of cells in MTT also significantly increased in a dose-dependent manner (Figure 2D). Both of them prove that the ANVs and THB@ANVs still retain the function of ADSCs in promoting cell proliferation. The effect of ANVs and THB@ANVs on other types of wound repair related cells is similar, which were detailed in Figure S9 and S10. No statistical difference was observed between ANVs group and THB@ANVs group, indicating that the addition of AIE molecules did not adversely affect the proliferation and migration of cells.