2.6.2 Histological analysis
During the remodeling phase, collagen deposition can indicate the
integrity of skin repair, with its ability to improve the tensile
strength and epidermal integrity of the tissue. To further find the
healing effect of the hydrogels on the wound tissues, the representative
H&E and Masson’s trichrome staining on days 7 and 14 were carried out
on the dissected wound tissues (Figure 6A). ANVs can enhanced collagen
deposition on the regenerated tissue sites. The quantified wound
macroscopic observations were in accordance with the closure rates. This
also confirmed the in vitro cell experiment results. Higher
expression of collagen in scald wound area was observed in ANV hydrogel
and THB@ANVs hydrogel group at day 7 and 14. Both data showed that the
wounds heal of THB@ANVs hydrogel group were significantly enhanced.