Scheme 1. Schematic illustration for the contrustion and deep
scald wound repairment mechanism of THB@ANVs encapsulated hydrogel, the
wound recovery process was presented from left to right.
In this study, we used easily accessible adult adipose-derived stem
cells to extrude mass-produced nanovesicles (ANVs), which were then
combined with photodynamic AIE nanoparticles of
4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)vinyl)-1-(2-hydroxyethyl)
pyridin-1-ium bromide (THB) to enhance their anti-infectious ability
(denoted as THB@ANVs). To encapsulate the THB@ANVs without altering
their active ingredients, we used a heat-, light-, and catalyst-free
crosslinking amino-yne click chemistry CMC-DA hydrogel composed from
amino-rich water-soluble carboxymethyl chitosan (CMC) and di-activated
alkyne-modified polyethylene glycol (PEG-DA)[30](Scheme 1). Our research demonstrated that this hydrogel can match the
shape of a wound through injection, and that the β -aminoacrylate
bond in the hydrogel can accelerate the release of contents in response
to the acidic environment of the wound. In vivo wound healing
evaluations are also conducted to investigate the effectiveness of this
comprehensive hydrogel for treating bacterial infections, promoting
wound tissue repair, and reducing treatment time.