4.4.5 Hemolysis test
The porcine red blood cells separated from the whole blood were gently
wash with 3 volumes of saline, then centrifuged at 500~1000 r/min for 5
minutes, repeating the process 2~3 times. After being
diluted 20 times, the red blood cells were incubated with an equal
volume of saline, deionized water, AIE EVs solution or hydrogels
immersed solutions at 37 ℃ for 3h. The supernatants were obtained by
centrifugating the mixture at 750g for 5min, and their absorbance at 545
nm will be measured. The hemolysis rates are calculated as the following
formula: Hemolysis rate (%) = (A-Asaline) /
(Awater - Asaline) ×100%. The hemolysis
rate exceeds 5% is considered hemolysis.