4.5 In vivo wound healing assessment in a deep Ⅱ° scald wound
molding
On 0 d, The SD rats weighing 180−200 g were anesthetized with inhaled
gas anesthesia (O2 2 L/min,2% isoflurane) before
surgery, and the hair on their back was shaved. To induce a deep Ⅱ°
scald wound, a preheated copper-block (500g) of a temperature control
scald instrument at the temperature of 95 °C for 15 s at the dorsal area
of each rat. Twelve rats were randomly divided into four groups with
different treatments: (1) Control group in which saline alone was
applied to the wounds, (2) blank hydrogel group, (3) ANVs hydrogel
group, (4) THB@ANVs hydrogel group. 2mm hydrogel in with the same area
as the wound were applied to cover the wounds of group (2)(3)(4).
On 1d, 60 μL of 106 S. aureus were dropped onto
the wound surface after debridement, then saline or hydrogel were
applied to the scald wound and covered by Tegaderm™ film dressing (3M
Deutschland GmbH, Neuss, Germany). After incubating the hydrogel on the
wound for 6 hours, photodynamic therapy was performed using 100
mW/cm2 white light source for 10 min. The sustained
release time of gel is 3 days, and the frequency of dressing change is
consistent with it. Photodynamic therapy was performed every day from
1~7d, and every other day from 8~14d,
and sampled on days 3, 7, 14 and 21 have been collected. The images of
wound healing regions were recorded on days 0, 1, 3, 7, and 14. The
wound areas and vascular regeneration were measured and analyzed by
Image J software.