4.4.6 Antibacterial ability assay of THB@ANVs
The bacteria imaging was carried by mixing 90 μL of bacteria with 10 μL
newly prepared THB@ANVs for 20 min at ambient temperature, then observed
on glass slides. The images were collected under FL microscope (100×,
excitation filter 528-553nm, dichroic mirror 565nm, emission filter
578-663nm). Scanning electron microscopy (SEM) was applied to observe
the morphologies of the bacteria before and after treated with THB@ANVs.
After the treatment above, the bacteria were fixed with 2%
glutaraldehyde at 4 °C overnight and then rinsed with water, followed by
dehydration with a sequential ethanol/water mixtures with an increasing
ethanol volume of 30%, 50%, 70%, 80%, 90%, 95%, and 100%,
respectively. Finally, the samples were dropped on a silicon wafer,
vacuum dried, and coated with platinum before observation.