4.4.4 Apoptosis analysis
The number of apoptotic and necrotic cells was analyzed by AM&PI
staining apoptosis detection kit. In brief, 2 × 105HaCaT cells were cultured overnight in 6-well plates. After removing the
culture medium, the cells were incubated in the extraction solution
obtained by soaking the hydrogels in culture medium for 24 h. The cells
were stained with AM (5 μl) and PI (5 μl), left in dark for 15 min then
observed by fluorescence microscopy.