2.1 Materials
PIC Ⅱ (PS0323) was obtained from PUSH BIO-Technology (Chengdu, China).
Mouse IL-2, mouse transforming growth factor-β (TGF-β) and mouse IFN-γ
(C746) were purchased from Novoprotein Co., Ltd. (Shanghai, China).
Lipopolysaccharide (LPS) and other consumables were obtained from
Sigma-Aldrich (St. Louis, USA). Antibodies against F4/80 (28463-1-AP),
collagen 1 (COL, Col1a1 ) (67288-1-AP), fibronectin (FN,Fn1 ) (15613-1-AP), C-X-C motif chemokine ligand 16 (CXCL16
(60123-1-lg), myeloperoxidase (MPO) (66177-1-Ig), Janus kinase 1 (JAK1)
(66466-1-lg), tyrosine
kinase 2 (TYK2) (67411-1-lg), PERFORIN (14580-1-AP), GZMB (13588-1-AP),
CD11B (66519-1- lg) and β-Actin (66009-1-Ig) were obtained from
Proteintech (Rosemont, USA). Antibodies against histone H3 (citrulline
R2/R8/R17) (ab5103) and CD161 (ab197979) were purchased from Abcam
(Cambridge, USA). Antibody against a-smooth muscle actin (α-SMA,α-SMA ) (19245S) was obtained from Cell Signaling Technology
(Danvers, USA). Antibodies against signal transducer and activator of
transcription 1 (STAT1) (A12075), p-STAT1 (AP0453), p-JAK1 (AP0530),
p-TYK2 (AP0543) and CD68 (A13286) were purchased from ABclonal
Technology Co.,Ltd. (Wuhan, China). Antibody against CXCR6 (E-AB-11112)
was obtained from Elabscience Biotechnology Co.,Ltd (Wuhan, China).
Alexa Fluor 594 anti-rabbit IgG secondary antibody (8889S) was purchased
from Cell Signaling Technology (Danvers, USA), and Alexa Fluor 488
anti-mouse IgG secondary antibody (A32723) was obtained from Thermo
Fisher Scientific (Waltham, USA).