2.5 Transwell assay of NK cells and macrophages
After extraction and concentration, a total of 1 × 105NK cells were added in the top chamber of the transwell systems (0.4 μm)
with 1% FBS DMEM medium, 1 × 105 attached macrophages
were added in the lower chamber of the transwell systems with 1% FBS
DMEM medium and treated with LPS (50 ng/mL), IFN-γ (2.5 ng/mL), and
different doses of PIC Ⅱ (5, 10, 20 mM) for 6 h (Fig. 4D ).