Fig 5. CM secreted by M1-type macrophages containing PIC Ⅱ facilitated
NK cells to promote the apoptosis of HSCs. (A) Flow chart of the primary
NK cells co-cultured with HSCs. (B) CCK-8 results. (C) Relative mRNA
levels of Acta2 , Col1a1 and Fn1 were measured by
qPCR and further normalized with Hprt1 in the co-culture cells.
(D) Flow cytometry of 7-AAD and a-SMA and (E) representative
images of immunofluorescence staining for α-SMA in the co-culture of
primary NK cells and HSCs. Scale bar = 100 μm. Statistical significance:
*P < 0.05, compared with the control group;#P < 0.05,###P < 0.001, compared with the
model group (n = 3).