Fig 6. PIC Ⅱ enhances the killing effects of NK cells on HSCs through IFN-γ-Jak1/Tyk2-STAT1 pathway. Relative mRNA levels of Ifn-γ were measured by qPCR and further normalized with Hprt1 in (A) mice and (B) primary NK cells. The protein levels of p-JAK1, JAK1, p-TYK2, TYK2, p-STAT1, STAT1 were measured by western blot and normalized by β-ACTIN in the nucleus and cytoplasm of (C) mice liver tissues and (D) co-culture system of NK cells and HSCs. Quantifications were performed using Image J. Statistical significance: *P < 0.05, compared with the WT group; #P < 0.05, ##P < 0.01,###P < 0.001, compared with the model group (n = 3).