Figure legends:
Fig 1. PIC Ⅱ alleviates hepatic fibrosis and inflammation in Mdr2−/− mice. (A) Flowchart of the animal experiment and body weight changes of different groups. (B)Representative images of H & E staining and Masson trichome’s staining. Quantifications were performed using Image J . Scale bar = 20 μm.(C) Serum levels of ALT, AST, TBA and AKP. (D) The heatmap and clusters of liver fibrosis-related markers. (E)Relative mRNA levels of Fn1 , Acta2, H19 ,Col1a1 , Mmp13 , and Timp1 were measured by qPCR and further normalized with Hprt1 . (F) Representative images of immunofluorescence staining for Fibronectin, α-SMA, and Collagen1 in the liver. Quantifications were performed using Image J. Scale bar = 20 μm. Statistical significance: *P < 0.05, **P< 0.01, ***P < 0.001, compared with the WT group; #P < 0.05,##P < 0.01,###P < 0.001, compared with the Mdr2-/- group (n = 6).