Fig 7. PIC Ⅱ inhibited the NETs formation and promoted the rupture of
aHSCs to alleviate liver fibrosis. (A) GSEA analysis of neutrophil
inhibition. (B) The heatmap and clusters of neutrophils-related markers.
(C) Representative images of immunofluorescence staining for MPO and
CitH3 in the mice liver. Quantifications were performed using Image J.
Scale bar = 20 μm. (D) MPO activity. (E) Relative expression of mRNA
levels of Ly6g , Icam1 and Cxcr4 in mice liver were
measured by qPCR and further normalized with Hprt1 .
Representative images of immunofluorescence staining for (F) MPO and
CitH3 in the primary neutrophils and for (G) α-SMA in the HSCs treated
with PIC Ⅱ and CM-Neu. Scale bar = 100 μm. Statistical significance:
*P < 0.05, ***P < 0.001, compared with
the control group; #P < 0.05,###P < 0.001, compared with the
model group (n = 3).