Fig 7. PIC Ⅱ inhibited the NETs formation and promoted the rupture of aHSCs to alleviate liver fibrosis. (A) GSEA analysis of neutrophil inhibition. (B) The heatmap and clusters of neutrophils-related markers. (C) Representative images of immunofluorescence staining for MPO and CitH3 in the mice liver. Quantifications were performed using Image J. Scale bar = 20 μm. (D) MPO activity. (E) Relative expression of mRNA levels of Ly6g , Icam1 and Cxcr4 in mice liver were measured by qPCR and further normalized with Hprt1 . Representative images of immunofluorescence staining for (F) MPO and CitH3 in the primary neutrophils and for (G) α-SMA in the HSCs treated with PIC Ⅱ and CM-Neu. Scale bar = 100 μm. Statistical significance: *P < 0.05, ***P < 0.001, compared with the control group; #P < 0.05,###P < 0.001, compared with the model group (n = 3).