Fig 8. Depletion of macrophages partially neutralizes the anti-fibrotic
effect of PIC Ⅱ. (A) Flowchart of the animal experiment. (B) Serum
levels of TBA and TBIL. (C) Representative images of H&E staining and
Masson’s trichome staining. Scale bar = 20 μm. (D) Representative images
of immunofluorescence staining for F4/80 and CXCL16 in the liver. Scale
bar = 20 μm. (E) Relative mRNA levels of Cxcl16 , Inos ,Fn1 and Col1a1 were measured by qPCR and further
normalized with Hprt1 . (F) The protein levels of FIBRONECTIN and
COLLAGEN were measured by western blot and normalized by β-ACTIN in the
liver. Quantifications were performed using Image J. Statistical
significance: *P < 0.05, **P < 0.01,
***P < 0.001, compared with the WT group;##P < 0.01,###P < 0.001, compared with the
Mdr2-/- group (n = 6).