Fig 8. Depletion of macrophages partially neutralizes the anti-fibrotic effect of PIC Ⅱ. (A) Flowchart of the animal experiment. (B) Serum levels of TBA and TBIL. (C) Representative images of H&E staining and Masson’s trichome staining. Scale bar = 20 μm. (D) Representative images of immunofluorescence staining for F4/80 and CXCL16 in the liver. Scale bar = 20 μm. (E) Relative mRNA levels of Cxcl16 , Inos ,Fn1 and Col1a1 were measured by qPCR and further normalized with Hprt1 . (F) The protein levels of FIBRONECTIN and COLLAGEN were measured by western blot and normalized by β-ACTIN in the liver. Quantifications were performed using Image J. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, compared with the WT group;##P < 0.01,###P < 0.001, compared with the Mdr2-/- group (n = 6).