2.5 Blood pressure and heart rate detection
The Dataquest ART system by Data Sciences International was utilized for
real-time monitoring of blood pressure and heart rate for 24 hours on
day 1, 7, 14, 21, and 28 after administering, with 11:00 AM data serving
as a baseline each day (refer to Figure 1). The analysis of blood
pressure was performed using Ponemah Software 5.0 from Data Sciences
International, and the MAP (Mean Arterial Pressure) was
calculated using the following
formula: MAP = (SBP + 2*DBP) / 3.
2.6 Water
consumption, natriuresis and diuresis detection
Water consumption and urinary output were measured using a metabolic
cage. Natriuresis and diuresis were assessed on day 1, 4, and 28, 6
hours after dosing. Natriuresis was quantified using
Na+ test kits and a fully automatic biochemical
instrument from HITACHI.