Intro: Removal of bacterial plaque and stains are a crucial part of non-surgical periodontal treatment. Following scaling, polishing by air-powdered devices is widely used. The aim of this study is to evaluate the changes caused by three different air-powder abrasives from the same company (sodium bicarbonate, glycine, and erythritol) on the enamel and exposed root surface. Methods: The enamel and exposed cementum surface were air polished at an instrumentation time of 5s, combinations of medium and maximum power, medium water settings, the distance of 5mm, and angulation of 60 ⁰. Samples were scanned in a micro-computed tomography (micro-CT) at baseline and then after air-polishing powder applications, and the defect depth, defect volume, demineralization depth, and tissue mineral density (TMD) values were evaluated. Results: Sodium bicarbonate-based powder is more abrasive than glycine and erythritol-based powders ( p<0.05). All powders caused more abrasion at the maximum power setting ( p<0.05). There were statistically significant differences between the mean TMD values before and after the application in all groups ( p<0.05). After the application, the average TMD was found to be lower. Conclusion: We can conclude that glycine and erythritol-based powders can be used for supragingival air polishing in patients with gingival recession, in addition, we can recommend that the power setting be set to the minimum level at which stain, and plaque can be effectively removed.