Flow cytometry assay
For cell cycle analysis, cells treated with or without PDS were
harvested and fixed in ice-cold 70% ethanol, stained with 1X PBS based
propidium iodide solution (50μg/mL PI, 100μg/mL RNase A, 0.1%
sodium citrate, 0.1% Triton X-100), and analyzed by flow cytometry
(Agilent).