Cell culture and treatment
All mouse protocols have been approved by the Animal Care and Use
Committee of Guizhou Medical University. The MEF cells of WT (wild type)
and G3DKO (G3mTR-/-mTerc-/- )
were harvested in e13.5 days and cultured in Dulbecco’s Modified Eagle
Medium (DMEM) with 10% fetal bovine serum (FBS) at 37℃ with 5%
CO2 and 3% O2. The MEFs were used for
experiments before passage 5 and the cells were passaged at a ratio of
1:2 or 1:3.
The cultured cells were treated with Bazhen decoction (control, low dose
(10μg/mL), high dose (20μg/mL)) for 48hr. For G4 structure stabilizer
treatment, the cells were treated with 5μM pyridostatin hydrochloride
(PDS) (MCE) for 24h. For combined treatment of Bazhen decoction and PDS,
the cells were treated with Bazhen decoction (control, low dose
(10μg/mL), high dose (20μg/mL)) for 24hr, then add 5μM PDS and continue
the treatment for another 24hr.