A. Spike protein mutation sites of SARS-CoV-2 Prototype (Genebank ID: MN908947), Omicron variants. BA.1 and BA.5. B.The IgG titers against S protein of SARS-Cov-2 prototype, Omicron variants BA.1 and BA.5. The limitation of the detection (LOD) was1:400. The titers below the LOD were set to 200.C. The neutralizing antibody titers against Prototype, and Omicron variants BA.1 and BA.5 in individuals post the 3rd booster vaccination within 2 months. The limitation of the detection (LOD) was 1:10. The titers below the LOD were set to 5.P -values were obtained by the unpaired t test, *** P < 0.001, **** P < 0.0001, ns, not significant.