Figure 2. Meglumine (ME) causes mast cell (MC) activation without affecting viability. Circular diagram showing the composition of meglumine gadoterate (MeGa) (A) . LAD2 cell viability and activation was assessed by flow cytometry following stimulation with ME or vehicle control (-) (B). LAD2 cells were stimulated with increasing concentrations of ME and MeGa to determine the half maximal effective concentration (EC50) of LAD2 cell activation(C) . Pooled data from 10-12 (B) or 3-5 (D) independent experiments represented as mean ± SEM, *p≤0.05; **p≤0.01; ***p≤0.001.