Materials and Methods
Sample
collection
Samples of fish eggs and larvae were collected from 30 stations between
121.5°~124°E and 29.5°~31°N (Fig. 1)
based on the Technical Regulations of Marine Biological Ecological
Investigation in the ECS-CZA in April and November of 2018, and April
and November of 2019. Sample collections used horizontal opening trawls
(opening diameter in 80 cm; net opening area of 0.5m2;
net length of 280 cm). Trawling used a constant speed of 2km for about
10 minutes. All the sample collection locations were shown in Fig 1. The
planktonic samples were sieved through smaller sized meshes, washed with
seawater, and then fish eggs and larvae were sorted and separated from
the plankton samples and placed into different jars with 100% ethanol
according to sampling stations and seasons.