Taxon Sampling and Laboratory Procedures
To provide a comprehensive phylogeny of H. leucisculus , fishes were obtained from four different recorded localities in Iran containing Karun River, Mahabad Dam, Chitgar artificial lake and gorganrud (Fig.1). The fishes were euthanized and clips of left pectoral fins were removed and then preserved in 96% ethanol. Collection and locality data for sampled fishes are described in Table. S1.