Reconstruction of Phylogeny
In the current study, to investigate the situation of individuals belonging to the H. leucisculus species, a phylogeny tree was constructed based on 14 sequences studied and 84 sequences from the native distribution area of the species. The datasets, with 617 bp length included partial sequences of cytb.
The Bayesian topology inferred by partitioned analysis was adopted for explaining the H. leucisculus phylogeny. The constructed intra- species phylogenetic trees from cytb showed the same topologies for both ML and BI trees. Relative to the outgroup, the Hemiculter bleekeri clustered as sister monophyletic lineages. The phylogenetic trees demonstrate that the H. leucisculus group are well separated from each other with high support values. According to the Phylogenetic tree, the H. leucisculus group was considered a well-supported monophyletic group as expected, and three distinct clades were revealed, the Iranian samples belonged to the 2rd clade (Fig. 2). The samples of this study belong to the china catchment clade and mainly the Yangtze River, which indicates the origin of the individuals studied in Iran.