Reconstruction of Phylogeny
In the current study, to investigate the situation of individuals
belonging to the H. leucisculus species, a phylogeny tree was
constructed based on 14 sequences studied and 84 sequences from the
native distribution area of the species. The datasets, with 617 bp
length included partial sequences of cytb.
The Bayesian topology inferred by partitioned analysis was adopted for
explaining the H. leucisculus phylogeny. The constructed intra-
species phylogenetic trees from cytb showed the same topologies for both
ML and BI trees. Relative to the outgroup, the Hemiculter
bleekeri clustered as sister monophyletic lineages. The phylogenetic
trees demonstrate that the H. leucisculus group are well
separated from each other with high support values. According to the
Phylogenetic tree, the H. leucisculus group was considered a
well-supported monophyletic group as expected, and three distinct clades
were revealed, the Iranian samples belonged to the 2rd clade (Fig. 2).
The samples of this study belong to the china catchment clade and mainly
the Yangtze River, which indicates the origin of the individuals studied
in Iran.