Figure 1. Integration of information on Bacillus subtilisgenes and proteins in the database SubtiWiki (http://subtiwiki.uni-goettingen.de).
  1. Basic information on the highly expressed unknown protein YjlC. The gene-specific page includes all available information on the protein as well as graphical interactive elements for the genomic content, the structure of the protein, the expression of the gene under more than 100 conditions, protein-protein interactions, and regulation of the gene. Note that the yjlC and ndh genes form an operon, and the encoded proteins interact physically (see also Table 1) indicating a functional link between the two proteins.
  2. The Subti Wiki category for highly expressed understudied proteins give immediate access to the set of 41 proteins, sorted according to potential functions (see Table 1).
  3. The Expression Browser of SubtiWiki allows a direct comparison of the expression levels of two or more genes (here yjlC andndh ). Similar expression patterns support the idea of a functional link between two genes/proteins.
Figure 2 . Overview of transporter proteins with unknown function and amino acids with no known transporter . While some of these proteins are only expressed during sporulation, others are moderately expressed under standard growth conditions. For five of twenty proteinogenic amino acids no transporter responsible for the uptake of the particular amino acid could be identified. The existence of these transporters is still guaranteed, as mutants of the synthesis genes for these five amino acids are viable in complex medium.