Exposure of Control and Pesticide-selected Populations to
Pesticide and Bt Oral Diets
After eight generations of selection, we generated same-age F2 larvae
from pesticide-selected and susceptible colonies. Approximately 200
adults from Gen. 8 pesticide-selected and susceptible control colonies
were allowed to reproduce on control diet for three days. We removed
these F0 adults and allowed offspring to develop to F1 adults. To
generate F2 experimental larvae, we placed 4-5 replicates of 80 adults
per selection line in 100 mm petri dishes with control diet and allowed
them to reproduce for 24 hours. F2 larvae were allowed to develop for 12
days.
We placed twelve day old larvae (~4 mm long) on oral
diet plates prepared as described in the previous section (Suppl. Fig.
1; n = 96/ population/ treatment). Half of the plate (n = 48) was
monitored daily for three weeks for survival and development.
Individuals in the remaining half were sampled after two days of
exposure prior to the onset of mortality and used for RNAseq analyses
(described below).