Quality assessment
We referred to a previously published study in assessing the study quality.21 WT (University of North Carolina at Chaple Hill) is the team’s leading HSV-2 assay analysis assessment expert. Study precision was categorized into high and low based on the sample size (low: <200 vs. high ≥200). The risk of bias was assessed based on the sampling method (probability-based vs. non-probability-based) and response rate (low: <80% vs. high ≥80%). We consider studies using existing medical records as non-probability-based studies and of high response rates.