2.4 Urinary biomarkers
Urine samples were analyzed using MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panel 1 (EMD Millipore Corporation, Charles, MO) according to the manufacturer’s protocol (Pais, Liu, Avedissian, et al., 2020). MILLIPLEX® Analyst v5.1 Flex software was used for calibration curve and analyte concentration determination.