2.3 Sample collection
Blood samples (0.125 mL, maximum 16 samples/animal) were drawn from a jugular vein catheter into EDTA-containing tubes, centrifuged at 3000 rpm for 10 min, (O’Donnell et al., 2017; Rhodes et al., 2016) and the resultant plasma was stored at -80oC for batch analysis. Blood was sampled daily under the #3151 protocol and each day except Day 3 under the #3080 protocol. Blood sampling was carried out for a pharmacokinetic study that will ultimately be detailed elsewhere. Urine was collected continuously, aliquoted every 24 h, centrifuged (500xg, 10 minutes at 4°C), and supernatant was stored at -80oC until batch analysis. At the end of the protocol, animals were anesthetized with ketamine/xylazine (100/10 mg kg-1, by intraperitoneal injection) and euthanized by exsanguination through the right atrium while under anesthesia. Kidneys were obtained at euthanasia, rinsed in cold saline, flash frozen and stored at -80⁰C for vancomycin assay.