2.3 Sample collection
Blood samples (0.125 mL, maximum 16 samples/animal) were drawn from a
jugular vein catheter into EDTA-containing tubes, centrifuged at 3000
rpm for 10 min, (O’Donnell et al., 2017; Rhodes et al., 2016) and the
resultant plasma was stored at -80oC for batch
analysis. Blood was sampled daily under the #3151 protocol and each day
except Day 3 under the #3080 protocol. Blood sampling was carried out
for a pharmacokinetic study that will ultimately be detailed elsewhere.
Urine was collected continuously, aliquoted every 24 h, centrifuged
(500xg, 10 minutes at 4°C), and supernatant was stored at
-80oC until batch analysis. At the end of the
protocol, animals were anesthetized with ketamine/xylazine (100/10 mg
kg-1, by intraperitoneal injection) and euthanized by exsanguination
through the right atrium while under anesthesia. Kidneys were obtained
at euthanasia, rinsed in cold saline, flash frozen and stored at -80⁰C
for vancomycin assay.