2.6 Materials
Treatments were clinical grade vancomycin (Fresenius Kabi, Lake Zurich,
IL), flucloxacillin (Juno Pharmaceuticals Pty Ltd, South Yarra, VIC
Australia), imipenem-cilastatin (Novation LLC, Italy Novaplus/Novation
Mfd. Fresenius Kabi) and normal saline (Veterinary 0.9% Sodium Chloride
Injection USP, Abbott Laboratories, North Chicago, IL).
For LC-MS/MS, analytical grade vancomycin hydrochloride (Lot#LRAC0718),
and atenolol (Lot#BCBT5643) were obtained from Sigma Aldrich (St.
Louis, MO), LC-MS/MS grade acetonitrile and methanol were obtained from
VWR International, Radnor, PA, formic acid was obtained from Fisher
Scientific, Waltham, MA and frozen, non-medicated, non-immunized, pooled
Sprague–Dawley rat EDTA plasma was obtained from BioreclamationIVT,
Westbury, NY, USA. Control rat kidney homogenate was prepared from
saline-treated rat kidneys.