2.7 p38 transfection
The p38-overexpressing lentiviruses (LV-p38) were generated by transfecting third-generation viral packaging plasmids (pRSV-Rv:VSVG:pMDLg/RRE, 5:3:2) and lentiviral vectors (p38-Flag) into HEK 293T cells. Briefly, 5×105 HEK 293T cells were co-transfected by 2 μg packaging plasmids and 2 μg lentiviral vectors. The virus supernatant was collected after 48 h transfection. N2a-sw cells were then incubated in the virus supernatant for 36 h, following selection with puromycin (Invivogen; 2 μg/ml). Generation of N2a-sw cells overexpressing p38-Flag was confirmed by western blotting.