2.12 Sandwich ELISA
For the detection of Aβ42 in the N2a-sw cell medium, the cell mediums were collected after the treatment of Lf (0.05 mg/ml and 0.1 mg/ml) for 24 h. The Aβ42 content was determined using Aβ42 kits (Invitrogen, HKB3544) according to the manufacturer’s instructions. Absorbance values were recorded using a microplate reader at a wavelength of 450 nm.