2.8 LRP1 knockdown
The mouse LRP1-specific sense (5’-GGA GUC ACU UAC AUC AAU AUU-3’) and antisense (5’-UAU UGA UGU AAG UGA CUC CUU-3’) RNA oligonucleotides were synthesized by Hanbio Biotechnology Co., Ltd (Shanghai, China). The LRP1 siRNA were generated according to the manufacturer’s instructions. Cells were transfected with LRP1 siRNA (50 nM) using GP-Transfect-mate (GenePharma, G04008) according to the manufacturer’s specifications. Cells were further cultured for 48 h before treating with Lf (0.1 mg/ml) for 24 h.