2.4 Aβ42 oligomer preparation
The Aβ42 oligomer was generated as our previous study (Fan et al., 2019). Briefly, the lyophilized Aβ42 peptide (ChinaPeptides, China) was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP; Sigma, 105228) and divided into quarters before removing HFIP. The Aβ42 oligomer was obtained by incubating at 4 ℃ for 24 h in the F12 medium. Western blotting using the antibody against Aβ oligomer (Millipore, AB9234, 1: 1000) was performed to the quality control of the Aβ42 oligomer.