Figure 1. Overexpression of Lf in astrocytes reduces Aβ burden
and improves cognitive ability in APP/PS1 mice. The male mice at
10-months-old were submitted to MWM tests with 2 days of visible
platform training, and 5 days of hidden platform tasting, as well as a
probe trial after 24 h of the last hidden platform test. The numbers of
mice used in this study as follow: WT = 8, Astro-Lf = 8, APP/PS1 = 8,
APP/PS1/Lf = 8. (A-B) The escape latencies and path lengths in the
visible platform tests. (C-D) The escape latencies and path lengths at
day 5 in the hidden platform tests were shortened in the WT mice and
APP/PS1/Lf mice, compared to those in APP/PS1 mice. * showing WTvs APP/PS1, & showing APP/PS1 vs APP/PS1/Lf; Two-way
ANOVA followed by the Bonferroni’s post hoc test. (E) Graphs showing
representative paths at day 5 in the hidden platform tests. (F) In the
probe trials, the WT mice and APP/PS1/Lf mice exhibited more crossing
times than APP/PS1 mice. Two-way ANOVA followed by the Bonferroni’s post
hoc test. (G) Immunostaining showing the Aβ plaques in the mouse brains,
the magnified images of hippocampus and cortexes are respectively
presented in G1, G2 and G3, G4. n = 5. (H-I) Overexpression of Lf in
astrocytes decreased both the numbers and areas of Aβ plaque in APP/PS1
mice. n = 4; Student’s t -test. (J) The expression levels of Aβ
were reduced in APP/PS1/Lf mice compared to the APP/PS1 mice. n = 6;
Two-way ANOVA followed by the Bonferroni’s post hoc test.&P < 0.05,&&P < 0.01; *P <
0.05, **P < 0.01.