2.8 LRP1 knockdown
The mouse LRP1-specific sense (5’-GGA GUC ACU UAC AUC AAU AUU-3’) and
antisense (5’-UAU UGA UGU AAG UGA CUC CUU-3’) RNA oligonucleotides were
synthesized by Hanbio Biotechnology Co., Ltd (Shanghai, China). The LRP1
siRNA were generated according to the manufacturer’s instructions. Cells
were transfected with LRP1 siRNA (50 nM) using GP-Transfect-mate
(GenePharma, G04008) according to the manufacturer’s specifications.
Cells were further cultured for 48 h before treating with Lf (0.1 mg/ml)
for 24 h.