2.7 p38 transfection
The p38-overexpressing lentiviruses (LV-p38) were generated by
transfecting third-generation viral packaging plasmids
(pRSV-Rv:VSVG:pMDLg/RRE, 5:3:2) and lentiviral vectors (p38-Flag) into
HEK 293T cells. Briefly, 5×105 HEK 293T cells were
co-transfected by 2 μg packaging plasmids and 2 μg lentiviral vectors.
The virus supernatant was collected after 48 h transfection. N2a-sw
cells were then incubated in the virus supernatant for 36 h, following
selection with puromycin (Invivogen; 2 μg/ml). Generation of N2a-sw
cells overexpressing p38-Flag was confirmed by western blotting.