2.2 Tissue preparation
Mice were anesthetized with sodium pentobarbital (50 mg/kg, intraperitoneally), and subsequently transcardially perfused with physiological saline. Mice were sacrificed by decapitation, and the brains were immediately dissected in half on an ice-cold board. One was fixed in the 4% polyformaldehyde for morphological assessments, the other half was frozen at -80 °C for biochemical analyses.
Reagent
The hLf was purchased from Sigma-Aldrich (L4040), which was dissolved in physiological saline at the concentration of 25 mg/ml for the stock solution and stored at -80 °C.