AllergyScreen Test
Collect 2-3ml venous blood from the child, centrifuge for 10 at
3000r/min minutes, and take the serum supernatant. Inhaled allergen sIgE
levels were measured using the AllergyScreen test (Mediwiss Analytic
GmbH, Moers, Germany), an immunoblot assay that uses a uniform and
standardized procedure.
Results were interpreted into the following groups: grade 0
(<0.35KU/L), grade 1 (0.35~0.7 KU/L), grade 2
(0.7~3.5 KU/L), grade 3 (3.5~17.5 KU/L),
grade 4 (17.5~50.0 KU/L), grade 5
(50.0~100.0 KU/L), grade 6 (≥ 100.0 KU/L). The positive
is ≥ 0.35 KU/L.
Statistical method
SPSS 25.0 software was applied for statistical analysis. The positive
rate of allergens during and before COVID-19 was tested by the
Chi-square test. It could analyze the difference of allergens positive
rate in different subgroups by gender, season and age. Univariate
regression analyses were adopted to calculate the odds ratio (OR) and
95%CI for the associations between theCOVID-19 pandemic and allergen
sensitization. Multivariate regression analyses were used to calculate
the adjusted ORfor each allergen during the pandemic compared to that
before the pandemic, after adjusting confounding factors such as sex,
age, and season. P<0.05 was considered to have
statistical significance.