AllergyScreen Test
Collect 2-3ml venous blood from the child, centrifuge for 10 at 3000r/min minutes, and take the serum supernatant. Inhaled allergen sIgE levels were measured using the AllergyScreen test (Mediwiss Analytic GmbH, Moers, Germany), an immunoblot assay that uses a uniform and standardized procedure.
Results were interpreted into the following groups: grade 0 (<0.35KU/L), grade 1 (0.35~0.7 KU/L), grade 2 (0.7~3.5 KU/L), grade 3 (3.5~17.5 KU/L), grade 4 (17.5~50.0 KU/L), grade 5 (50.0~100.0 KU/L), grade 6 (≥ 100.0 KU/L). The positive is ≥ 0.35 KU/L.
Statistical method
SPSS 25.0 software was applied for statistical analysis. The positive rate of allergens during and before COVID-19 was tested by the Chi-square test. It could analyze the difference of allergens positive rate in different subgroups by gender, season and age. Univariate regression analyses were adopted to calculate the odds ratio (OR) and 95%CI for the associations between theCOVID-19 pandemic and allergen sensitization. Multivariate regression analyses were used to calculate the adjusted ORfor each allergen during the pandemic compared to that before the pandemic, after adjusting confounding factors such as sex, age, and season. P<0.05 was considered to have statistical significance.