2.1 Data collection
We systematically reviewed the studies related to copepod associated bacteriobiome. The relevant published research articles were searched and retrieved from PubMed, Google Scholar and SCOPUS using keywords such as copepods gut microbiome, copepod associated bacteria/microbiome, copepods gut flora, copepod microbiome and zooplankton associated microbiome on Jan 30th, 2020. Apart from the search for the published research articles, we also searched in public databases (for published Ion Torrent, Pyro, and Illumina sequence data) such as the NCBI-SRA, ENA, DDBJ and Figshare using the above-mentioned keywords.
Overall, 11 study data were retrieved for meta-analysis (Table 1) containing 514 next-generation sequence libraries. We have pre-processed separately every individual file within the study and prepared the quality control (QC) report.