3.4. Differential abundance of CAB revealed through ANCOM
ANCOM results showed that a total of 23 CAB phyla, viz., Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chlorobi, Crenarchaeota, Cyanobacteria, Elusimicrobia, Euryarchaeota, Firmicutes, Fusobacteria, Gemmatimonadetes, GN02, OD1, [Parvarchaeota], Planctomycetes, Proteobacteria, SBR1093, Spirochaetes, [Thermi], TM6, Verrucomicrobia, and WPS-2 were significantly different between the copepod genera with W and Centred Log-Ratio (clr) statistics ranging between 40 to 30 and 53 to 2.6, respectively (Table S1). The 23-CAB phyla consisted of 32 classes, 78 orders, 145 families and 242 genera which were significantly different between the copepod genera (Supplementary File S1). From these 240 CAB genera, the top two percentile (the W and clr statistical values are given in the supplementary file S1) were chosen to explain the percentile compositional difference of CAB between the copepod genera.
CAB taxa, viz., Pseudomonas, Anaerospora, HTCC2207, Acinetobacter, Ochrobactrum family Cryomophaceae, Flavobacteriaceae and Methylobacteriaceae (W and clr-statistical values are provided in the supplementary File S1) were found in high percentile withinCalanus spp. (Figure 3).
Furthermore, from the ANCOM analysis, the CAB taxa, viz.,Paulinella, RS62, Candidatus Portiera, Planktotalea, Segetibacter, Octadecabacter, family Rhodobacteraceae and order Bacteroidales were found in high percentile within Acartia spp. (Figure 3). In the case of Centropages sp. the CAB genera likeAlteromonas, Pseudoalteromonas, Fluviicola, Oleispira, Ralstonia and family Colwelliaceae were found to be high percentile. In addition, Temora spp. appeared to have high percentile ofComamonas, Planctomyces, Flavobacterium, Synechococcus, Chryseobacterium and Nitrosopumilus. Only four CAB genera,Bradyrhizobium, Marinobacter, Photobacterium, and Vibrio, were significantly high in Pleuromamma spp. (Figure 3).