calculated using the P. nobilis haplotypes, evidencing overall sequences significant negative D in 7 out of the 14 loci studied, which indicated, as well, an excess of low-frequency haplotypes relative to expectation (Table 4).
Noteworthy, however, analysis of regional Tajima’s D along the sequences revealed that TLR-1 and TLR-2 had regions associated with significant positive Tajima’s D (D>2 with p< 0.05 or p<0.01), indicating a balancing selection acting on part of these proteins. Specifically, these observations concerned LRR at the extra-cellular domains (two and one regions for TLR-1 and TLR-2 respectively) and one region of the TIR domain of TLR-1 (data not shown ). We could not evidence any sequence variations linked to either “Resistant” or “Sensitive” groups for both TLR-1 and TLR-2 (Chi² test; df = 13; p-value = 1).