Twilight Photometer observations were carried out at low latitude station Kolhapur (16⁰39’42.2” N, 74⁰14’20.8” E) India, during the period 1 January 2009 to 31 December 2011 to yield a reasonable qualitative picture of the day-to-day variability of the vertical distribution of the atmospheric aerosols from about 6 km to a maximum of 350 km. In this study an attempt was made to observe twilight airglow red lines using the vertical profiles of aerosols. The rate of change of red light due to the twilight airglow shows narrow peaks with ~ 600-1000% rise in intensity than due to aerosol twilight glow. The principal aspiration of this study is to highlight the conspicuous capability of Semiautomatic twilight photometer to study the twilight airglow emission lines. Twilight Photometer, although currently seldom used, is still a very effective and the most appropriate ground based passive remote sensing tool for long-term monitoring of different atmospheric components in a wide range of altitude for day-to-day basis. It is an efficient system having a simple and inexpensive underlying principle of operations, yet exhibits extremely accurate and precise usage and can be operated by even a person of average skill. However, it can be seen that the resultant data was consistent with nearest peer technologies, such as LIDARs, Balloon-Borne instruments, rocket measurements, satellite observations etc. It is the first attempt in India to obtain the twilight airglow data using Twilight photometer.