3.5 NVP-BHG712 did not affect the expression of osteoclast differentiation-related receptors.
To further investigate whether NVP-BHG712 affected the expression of receptors related to osteoclast differentiation and thus affected the downstream signal in the cells, we observed the expression of the c-Fms receptor and RANK receptor in BMMs-derived osteoclasts by immunofluorescence assay. The immunofluorescence of the c-Fms receptor did not change significantly after treatment of osteoclasts with NVP-BHG712 at a concentration of 0.4 μM (Fig. 5I, J). In addition, the immunofluorescence results showed that NVP-BHG712 had no significant effect on the expression of the RANK receptor, which is related to osteoclast differentiation (Fig. 5K, L).