3.5 NVP-BHG712 did not affect the expression of osteoclast
differentiation-related receptors.
To further investigate whether NVP-BHG712 affected the expression of
receptors related to osteoclast differentiation and thus affected the
downstream signal in the cells, we observed the expression of the c-Fms
receptor and RANK receptor in BMMs-derived osteoclasts by
immunofluorescence
assay.
The immunofluorescence of the c-Fms receptor did not change
significantly after treatment of osteoclasts with NVP-BHG712 at a
concentration of 0.4 μM (Fig. 5I, J). In addition, the
immunofluorescence results showed that NVP-BHG712 had no significant
effect on the expression of the RANK receptor, which is related to
osteoclast differentiation (Fig. 5K, L).