Soft markers were divided in three groups: 1 (fetuses with one soft marker), 2 (fetuses with two soft markers), and ≥ 3 (fetuses with more than two soft markers). For group 1, soft markers were further divided into 12 isolated soft markers. Each subgroup was compared with fetuses with no identifiable anomalies and maternal age ≥ 35 years. pCNV: pathogenic/likely pathogenic copy number variations. OR: odds ratio; CI: confidence interval. Binomial test with bonferroni correction was to compare yields between groups. Bold values denote statistically significant at the P value < 0.05 level.